WebJan 10, 2024 · Our results showed that 60-min 70 μM BFA treatment, in the presence of a protein synthesis inhibitor, cycloheximide (CHX, ... c BiFC assays in N. benthamiana leaf epidermal cells show interactions of PRAF4 (left) or PRAF8 (right) with GNOM. nYFP N-terminal YFP, cYFP C-terminal YFP. Complemented YFP signals were converted to the … WebCycloheximide chase assays are an experimental technique used in molecular and cellular biology to measure steady state protein stability. Cycloheximide is a drug that inhibits the elongation step in eukaryotic protein …
Cycloheximide solution, 100mg/mL in DMSO, 0.2 ... - Fisher Scientific
WebJan 1, 2015 · Here we present a full cycloheximide chase assay in our laboratory using a lung adenocarcinoma cell line, CL1-5, as a model. Materials and Reagents Cell lines CL1-5/Flag-wtSlug stable cells CL1-5/Flag-4SA stable cells) Antibodies Flag M2 antibodies (Sigma-Aldrich, catalog number: F3165) β–actin antibodies (Sigma-Aldrich, catalog … WebOct 19, 2012 · (A) Cells were treated with cycloheximide (CHX), EGF or UV for the indicated time and analyzed by Western immunoblotting with anti-phospho-p38. (B and C) Flow cytometry analysis of CHX-induced internalization of EGF receptor with or without pretreatment with the p38-specific inhibitor SB203580. rickman castle
Frontiers Ubiquitin-Like Protein UBD Promotes Cell Proliferation …
WebAs causes for the removal of the symbiotic algae, the following possibilities can be considered: (1) dilution of the number of algal cells by the host's cell division in the presence of cycloheximide and (2) digestion of algal cells by … WebCycloheximide (CHX) (Sigma Aldrich) is stored as a 500 μg/mL stock solution, solved in dimethylsulfoxide (DMSO) at − 20°C in small aliquots. Proteasome inhibitor MG132 (Enzo Lifesciences) is prepared as a 10 mM stock solution in DMSO and stored in 30 μL aliquots at − 20°C.Final working concentrations are 50 μg/mL (1:1000) for CHX and 10 μM (1:1000) … WebAug 9, 2024 · We then investigated the effects of TRIM28 on NLRP3 protein degradation by cycloheximide (CHX) chase assay. Trim28 deficiency markedly promoted NLRP3 protein degradation (Fig. 4d, e ).... rickmancing the stone quotes