WebSep 12, 2024 · 查找了一下,列名分别为: qseqid query (e.g., unknown gene) sequence id; sseqid subject (e.g., reference genome) sequence id; pident percentage of identical matches; length alignment length (sequence overlap); mismatch number of mismatches; gapopen number of gap openings; qstart start of alignment in query; qend end of … WebMay 8, 2024 · # Run DIAMOND with blastx # Output format 6 produces a standard BLAST tab-delimited file $ {diamond} blastx \ --db $ {dmnd} \ --query "$ {fasta}" \ --out "$ …
Thread: [Trinotate-users] Loading blastp/blastx output to sqlite ...
WebJan 23, 2024 · As part of annotating the transcriptome assembly from the MEGAN6 C.bairdi taxonomic-specific reads, I need to run DIAMOND BLASTx to use with Trinotate.. Ran DIAMOND BLASTx against the UniProt/SwissProt database (downloaded today) on Mox. SBATCH script (GitHub): 20240123_cbai_diamond_blastx_megan.sh WebMay 19, 2024 · As part of annotating cbai_transcriptome_v3.0.fasta from 20240518, I need to run DIAMOND BLASTx to use with Trinotate. greatest shows in tv history
output both daa and outfmt 6 in the same run? #665
WebAug 14, 2024 · # Run DIAMOND with blastx # Output format 6 produces a standard BLAST tab-delimited file $ {programs_array [diamond]} blastx \ --db $ {dmnd} \ --query "$ {transcriptomes_array [$fasta]}" \ --out "$ {transcriptome_name}" .blastx.outfmt6 \ --outfmt 6 \ --evalue 1e-4 \ --max-target-seqs 1 \ --block-size 15.0 \ --index-chunks 4 done WebI wonder if there is a way to output daa (for MEGAN) and outfmt6 at the same time? I will also need the taxonomy info (ie. staxids sskingdoms skingdoms sphylums … WebSep 3, 2024 · hi, when you run TransDecoder.LongOrfs on the Trinity.fasta file, it should have created a directory: Trinity.fasta.transdecoder_dir Once you locate that directory, be sure to run the TransDecoder.Predict step in the working directory that contains that Trinity.fasta.transdcoder_dir/ within it. greatest shows of the 21st century