WebambientContribNegative 7 If features is a list, it is expected to contain multiple sets of mutually exclusive features. Each cell should only express features in at most one set; … WebAug 7, 2024 · Technically, downsampling on the reads is more appropriate as it recapitulates the effect of differences in sequencing depth per cell. This can be achieved by applying the downsampleReads function to the molecule information file containing the read counts: set.seed ( 100) no.sampling <- downsampleReads (mol.info.file, prop= 1) …

downsampleReads error · Issue #50 · …

WebambientContribNegative 7 If features is a list, it is expected to contain multiple sets of mutually exclusive features. Each cell should only express features in at most one set; no cell should express features in different sets. WebCan't run downsamplereads or read10xMolInfo on my h5 file. I have tried using different versions of DropletUtils (including most up to date) in in both R 4.0.2 and R 3.6.1. In … linguahouse staying at a hotel

Package ‘DropletUtils’ - TU Dortmund

WebdownsampleReads <-function (sample, prop, barcode.length = NULL, bycol = FALSE, features = NULL, use.library = NULL) # Downsamples the reads to produce a matrix of UMI counts, given # a HDF5 file containing … WebbarcodeRanks: Calculate barcode ranks chimericDrops: Remove chimeric molecules controlAmbience: Ambient contribution from controls defaultDrops: Call cells from number of UMIs downsampleReads: Downsample reads in a 10X Genomics dataset emptyDrops: Identify empty droplets encodeSequences: Encode nucleotide sequences … WebFeb 7, 2024 · downsampleReads: Downsample reads in a 10X Genomics dataset; emptyDrops: Identify empty droplets; emptyDropsCellRanger: CellRanger's emptyDrops … linguahouse stoicism