WebMay 20, 2015 · We recently published a study in which we tested this model using genetic lineage tracing with the Fezf2 and Cux2 loci. This work demonstrated that: (1) Fezf2-expressing (Fezf2 +) RGCs are multipotent and sequentially generate deep and upper layer cortical projection neurons followed by macroglia and (2) RGCs marked by Cux2-CreER … WebDec 22, 2024 · Here, we show that aIC neurons expressing Fezf2 (aIC Fezf2), which are the pyramidal tract neurons, signal motivational vigor and invigorate need-seeking behavior through projections to the brainstem nucleus tractus solitarii (NTS). aICFezf2 neurons and their postsynaptic NTS neurons acquire anticipatory activity through learning, which …
(PDF) Acetylcholine deficiency disrupts extratelencephalic …
WebTg (Fezf2-cre/ERT2)#Bche. Mutation details : The transgene was constructed from RP23-141E17 BAC with mutation of the translation initiation site to prevent expression of Fezf2 … WebThe Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at … fema is 350 answers
Cux2-Positive Radial Glial Cells Generate Diverse Subtypes of
WebJun 3, 2024 · Having verified the functionality of the Fezf2-CreER line, we bred it with mice carrying a conditional kinase activated (ka)Braf knock-in allele (lsl-kaBraf) (10, 21) to assess the ability of B-RAF gain-of-function to promote CST axon elongation after SCI.Expression of kaB-RAF was induced in 5 – 6 weeks old lsl-kaBraf : lsl-tdTom : Fezf2-CreER mice … WebFeb 15, 2024 · 该实验使用靶向PT神经元的Fezf2-CreER小鼠和靶向IT神经元的PlexinD1-CreER小鼠，这两种小鼠品系会和Ai162繁育，使得钙指标GCaMP6s在不同的PyNs中特异性表达（图1.a,b）。此外，还使用在所有PyNs中非特异性表达GCaMP6s的EMX小鼠。 ... WebJan 23, 2024 · Pyramidal tract and intratelencephalic neurons show distinct cortex-wide activity patterns. We used CreER lines to measure the activity of two developmentally distinct PyN types: Fezf2-CreER targeting PT neurons and PlexinD1-CreER targeting IT neurons 26, crossed with Ai162 mice 29 to achieve PyN-type-specific expression of the … definition of pragmatism in philosophy