Gfap-creert2
WebGFAP promoter and CreERT2) into mouse embryonic stem cell [16]. For genetic confirmation, we designed two unique sets of primers, each target- ing the promoter and transgene, and performed... WebMar 28, 2024 · Cre ERT2 cDNA was placed under the control of the human glial fibrillary acidic protein promoter. ( J:121777 ) Recombinase. activity. Activity: Tissue activity of …
Gfap-creert2
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WebThe GFAP gene provides instructions for making a protein called glial fibrillary acidic protein. This protein is a member of the intermediate filament family of proteins. … WebSep 1, 2011 · To avoid the contribution of GFAP + progenitors to gut neurogenesis during fetal/neonatal development and to directly test whether GFAP + cells give rise to neurons in the adult gut, we studied GFAP-CreERT2;Rosa-loxpEYFP mice. In these mice, Cre recombinase activity can be induced in GFAP-expressing cells by tamoxifen administration.
WebFor in-depth product & services help, ask our Technical Information Scientists WebJan 22, 2024 · Line#16 (GFAP-CreERT2) contains a 2.2-kb 5′ flanking sequence derived from the human GFAP gene. This promoter was shown to direct reporter lacZ expression to astrocytes in the CNS in transgenic mice, as well as direct astrocyte-specific transcription in cultured human cells (Brenner et al. 1994). Given these data, the same promoter will …
WebDec 8, 2024 · Control Gfap-CreERT2+/-;Nhe1f/f (wild-type) mice displayed BBB damage (elevated endothelial transcytosis and intracellular vesicles) and persistent cerebral hypoperfusion in an ischemic stroke model (transient middle cerebral artery occlusion). In contrast, Nhe1 Astro-KO mice exhibited significantly less endothelial transcytosis and … WebApr 16, 2024 · To compare the transcriptomic profiles between normal and tumor tissues, we microdissected the ME region from three control and four Rax-CreERT2::Braf V600E mice at 2 months post tamoxifen ...
WebGFAP-Cre transgenic mice have been developed to achieve gene recombination in astrocytes, the most abundant cells of the central nervous system, with pivotal roles …
Web(D–F) GFAPCreERT2:DTA mice were i.p. injected with tamoxifen at days 1–5. (D) At days 5–8, mice exhibited a severe paralysis that rapidly pro- gressed to all four limbs and was accompanied by weight loss (mean ± SEM; n = 3–7/group; two-way ANOVA test). get ahead in life 意味WebThe Jackson Laboratory glial fibrillary acidic protein gfap creert2 mice. Glial Fibrillary Acidic Protein Gfap Creert2 Mice, supplied by The Jackson Laboratory, used in various … get ahead of myself 中文WebThe Glast-CreERT2 (Cre) mouse line shows highest Cre-mediated recombination in astroglial cells from the cerebellum, hippocampus, and olfactory bulb, with lower activity in other brain regions and the retina . ... For the quantification of gliosis, glial fibrillary acidic protein (GFAP)-stained areas showing obvious shapes of a cell body were ... get ahead in the world 意味WebGenetic confimation of hGFAP-CreERT2 mouse line and its tamoxifen inducible Cre expression. (A) Genetic map of hGFAP-CreERT2. (B) The primer sequences and PCR protocol of hGFAP-CreERT2 transgenic ... christmas information and historyWebI have a GFAP-CreERt2 mouse; hence want to verify transgenic expression exclusively in the brain but we are detecting endogenous Cre expression despite using Qiagen RNA extraction kit with DNase. get ahead new college swindonWebSep 7, 2024 · Here, Aldh1L1-CreER T2; GFP mice were injected with tamoxifen every 12 h (12 h) for five consecutive days and killed 28 days post-injection (dpi). (M,N) … christmas in forest city ncWeb012849 B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J These GCE transgenic mice have a human glial fibrillary acidic protein (hGFAP) promoter sequence directing expression of a Cre … christmas information report