Ggt1-cre

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August undefined, 2024

WebJan 29, 2024 · floxed37 mice with the Ggt1-Cre mice38. We found that BRAF deficiency (Figure 3A) did not influence normal renal function (Suppl. Fig. 5). Moreover, when the control and BRAFPT-/- ... isolated primary RTECs from BRAF floxed mice and carried out in vitro Cre-mediated deletion (Suppl. Fig. 6A). Vemurafenib treatment induced cell-death … WebSTOCK Tg(Ggt1-cre)M3Egn/J. Stock No: 012841. Protocol 19476: Separated PCR Assay - Tg(Ggt1-cre)M3Egn. Version 3. 0. Notes. This assay will NOT distinguish hemizygous …

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WebApr 27, 2024 · Renal fibrosis in wild type and PT-Sirt6KO (Sirt6 flox/flox; Ggt1-Cre +) mice was induced by UUO surgery. After seven days, histologic examination and Western blot analysis were performed to examine extracellular matrix (ECM) protein expression. We evaluated inflammatory cytokine and cell adhesion molecule expression after ureteral … WebSCHEDULE G-1 (Form 5471) (December 2024) Cost Sharing Arrangement Department of the Treasury Internal Revenue Service Attach to Form 5471. crye jpc black multicam

PTEN alleviates maladaptive repair of renal tubular …

http://www.informatics.jax.org/allele/MGI:3798834 WebMay 15, 2024 · Ggt1-Cre mice were provided by Eric Neilson, Northwestern University (Chicago, IL), and described previously . The Ggt1 promoter is predominantly expressed … WebApr 4, 2024 · Tg (Ggt1-cre)M3Egn - Renal & Urinary SystemRecombinase Activity Detail. The rat proximal promoter to Ggt1 was used in a transgene construct to drive the … crye jpc r

Proximal Tubular Cell–Specific Ablation of Carnitine …

WebNov 22, 2024 · We used an initial pair of GGT1-Cre primers shown in Figure 1B, which were 400 bp in size. PCR primer sequences are listed in Table 1. Murine model of cisplatin-induced nephrotoxic AKI. Cisplatin nephrotoxic AKI was induced in randomly distributed male and female Trip13 Stop/Stop and GGT1-Cre +/– Trip13 Stop/Stop mice (aged 8–12 … WebMar 1, 2024 · wcn23-0488 decoy receptor 2 mediates tubular maladaptive repair by regulating ketogenesis after acute kidney injury bulk clearance wholesalersWebFeb 5, 2012 · Here we show that the BMP receptor activin-like kinase 3 (Alk3) is elevated early in diseased kidneys after injury. We also found that its deletion in the tubular epithelium leads to enhanced TGF-β1-Smad family member 3 (Smad3) signaling, epithelial damage and fibrosis, suggesting a protective role for Alk3-mediated signaling in the kidney. crye jpc 3

"WebThe genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the ... " - Ggt1-cre

Ggt1-cre

Phosphatidylinositol 3-kinase signaling determines kidney size

WebNov 16, 2024 · Transgenic B6.Cg-Tg(Ggt1-cre)M3Egn/J mice that expressed Cre recombinase under the control of gamma-glutamyltransferase 1 (Ggt1) promoter … WebFeb 1, 2024 · Additionally, quantitative proteomics and validated studies showed HMGCS2, a key enzyme for ketone synthesis, was increased in GGT1-cre DcR2 f/f mice compared with WT. And the levels of urinary and serum β-hydroxybutyrate were higher in GGT1-cre DcR2 f/f mice, suggesting DcR2 affects the synthesis of β-hydroxybutyrate through …

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WebNov 27, 2024 · In Ggt1-Cre mice, Cre recombinase is expressed in RTECs 7–10 days after birth, and as a result Cre-mediated gene ablation is unlikely to influence normal renal development (32). Vgf PT−/− mice were indistinguishable from WT littermates and normal renal function was not evidently influenced by Vgf deficiency in RTECs ( Fig. S3 ). WebMar 7, 2024 · Mutation details: The rat proximal promoter to Ggt1 was used in a transgene construct to drive the expression of cre recombinase, followed by a termination …

WebIn this study, we crossed Trp53 and Pten conditional knockout mice with Ggt1-Cre mice to generate a Ggt1-Cre; Trp53 LoxP/LoxP; Pten LoxP/LoxP; YFP LoxP/LoxP (GPPY) … WebApr 21, 2024 · l Ggt1-Cre mice were crossed with ROSA mT/mG mice to generate transgenic mice that express membrane-localized EGFP in renal tubular epithelial cells. …

WebSoftware to open or convert GT1 files. You can open GT1 files with the following programs: GenuTax Standard by GenuSource Consulting Inc. Free File Viewer Pro. WebJan 13, 2024 · Transgenic B6.Cg-Tg(Ggt1-cre)M3Egn/J mice that express. cre under the control of Ggt1 promoter were purchased. from The Jackson Laboratory. The GeneBank Accession. Number for UCP2 is …

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WebMay 15, 2024 · In this study, we crossed Trp53 and Pten conditional knockout mice with Ggt1-Cre mice to generate a Ggt1-Cre; Trp53 LoxP/LoxP; Pten LoxP/LoxP; YFP … bulk clear phone caseWebGgt1 Cre Transgenic Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … crye hot weather combat shirtWebFeb 1, 2024 · Additionally, quantitative proteomics and validated studies showed HMGCS2, a key enzyme for ketone synthesis, was increased in GGT1-cre DcR2 f/f mice compared … bulk clear nail polishWebOct 12, 2024 · ﬂoxed mice (stock no. 013106, Jackson Laboratory) with Ggt1-Cre mice (stock no. 012841, Jackson Laboratory). We obtained mT/mG mice that express the cell membrane-targeted (mT), two-color ﬂuores-cent Cre reporter allele from Jackson Laboratories (stock no. 007676). mT/mG mice were bred with the Ggt1-Cre strain to … crye kneepad holderWebFeb 6, 2024 · Homozygous CrATloxP female mice (C57BL/6J) were bred to male γ-glutamyl transferase Cre mice (Tg-(Ggt1-cre)M3Egn/J, mixed Balb/cJ/C57BL/6 background; The … bulk clear plastic picture frames crye jpc mag insertWebNov 1, 2024 · Section snippets Mouse lines used and study approval. Fan1 loxP/loxP mice were bred with γGt1-Cre mice (#012841, Tg(Ggt1-cre)M3Egn/J) 28 to generate proximal tubule–specific Fan1 KO. γGt1-Cre expression in the proximal tubule was demonstrated by crossing γGt1-Cre mice with NuTRAP mice (Jackson Laboratories #029899, B6;129S6 … crye jpc 20 back panel