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Ggt1-cre小鼠

WebCre基因前，使其启动Cre酶的表达，从而介导lox 位点问特定基因DNA片段的敲除，当敲除过程完成 后，DNA片段重新结合，形成一个lox位点。 WebJan 13, 2024 · Transgenic B6.Cg-Tg(Ggt1-cre)M3Egn/J mice that express. cre under the control of Ggt1 promoter were purchased. from The Jackson Laboratory. The GeneBank Accession. Number for UCP2 is NM_011671.4 ...

基因敲除小鼠基础知识 - 简书

Web诱导型 Cre-loxP 系统介导的时间特异性基因敲除，通过调控给予诱导剂的时间人为操控基因敲除发生的时间。那么诱导型Cre有哪几种类型？ 诱导型Cre分类. 启动子激活型 . 通过诱导剂来调控Cre 重组酶的启动子活性。例如：四环素诱导型、干扰素诱导型。 配体诱导型 WebJan 21, 2024 · 它指的是在老鼠的基因组中插入了一个连接有mCherry的Cre片段，该片段在XCr1启动子的控制之下，只会在cDC1细胞表达。 表达时，mCherry和Cre会被内源酶切 … eastfield mall covid hours https://michaeljtwigg.com

012841 - Strain Details - The Jackson Laboratory

WebApr 5, 2014 · 目前已有的Kap-cre工具鼠有：Tg(Kap-cre)1IsaPax2：即pairedboxgene2，高量表达于脑组织和肾中，目前应用于肾组织条件性敲pax2-cre鼠主要有Tg(Pax2 … Web010611 B6.129P2(Cg)- Ighg1 tm1(cre)Cgn /J This strain expresses Cre recombinase from the endogenous Ighg1 , immunoglobulin heavy constant gamma 1, locus. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in the study of gene function in germinal center, class switched memory and … Web诱导型 Cre-loxP 系统介导了时间特异性基因敲除。. 通过对给予诱导剂的时间的控制，人为操控基因敲除的发生。. 诱导型Cre有哪几种类型？. 通过诱导剂来调节驱动 Cre 重组酶的启动子活性。. 例如：四环素诱导型、干扰 … culligan fairfield california

肾小管组织特异性CRE 工具鼠 - 北京 - 百奥赛图 - 豆丁网

Category:基因工程鼠目录 - pku.edu.cn

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Ggt1-cre小鼠

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http://www.larc.tsinghua.edu.cn/post/971/31 012841 STOCK Tg(Ggt1-cre)M3Egn/J These GT.cre transgenic mice express Cre recombinase in cortical tubular epithelium of the kidney and may be useful for generating conditional mutations to study polycystic kidney disease and renal fibrosis.

Ggt1-cre小鼠

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WebMar 19, 2024 · Cre小鼠作用的时间点是不可控的，其剪切效率比Cre ER高。. CreER会有leaky (泄露)的情况。. CreER的结果直接取决于tamoxifen诱导时间窗口的基因表达的情 … WebFeb 6, 2024 · Homozygous CrATloxP female mice (C57BL/6J) were bred to male γ-glutamyl transferase Cre mice (Tg-(Ggt1-cre)M3Egn/J, mixed Balb/cJ/C57BL/6 background; The Jackson Laboratory). Offspring heterozygous to CrATloxP were backcrossed to the CrATloxP mice. This cross produced offspring of which 50% were PTC-specific CrAT …

WebMar 7, 2024 · Mutation details: The rat proximal promoter to Ggt1 was used in a transgene construct to drive the expression of cre recombinase, followed by a termination sequence from human growth hormone. Cre expression was limited to cortical tubular epithelium of the kidney, and was first detected around 7 days of age. () WebMar 31, 2016 · Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn Creek Township offers …

WebFeb 7, 2024 · Cre重组酶会识别到Loxp序列并且修改Loxp之前的基因/序列 (取决于Loxp的方向，建议维基百科看详细介绍，此处重点)，经常用这个技术knockout gene。. 2. Cre用途. 一般这个基因重组技术会用到杂交。. 打个比方，父亲老鼠里的某特异性基因A (我们感兴趣的基 … Web004782 STOCK Tg(KRT14-cre)1Amc/J A congenic version of this strain is available as Stock No. 018964 . The K14-Cre transgene has a human keratin 14 promoter directing expression of Cre recombinase. These K14-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in the ectoderm and its derivatives (including the skin, …

Web我司资源库拥有500超种模型，包括cre工具鼠，神经系统疾病模型，代谢疾病模型，肿瘤免疫，荧光及报告鼠等多种模型。覆盖多个热门领域，有效缩短实验周期。

http://www.labmodel.cn/mod_product-view-p_id-4264.html eastfield industrial estate scarboroughWebMar 19, 2024 · 基因敲除小鼠基础知识. 持续活化的Cre敲除基因的效率较高，但敲除基因的时间点不可控，导致基因敲除可能发生在发育早期或非预期的实验时间窗 。. 诱导型CreER虽具有时间可控性，提高了遗传操作的精确度，但敲除基因效率较低。. 为了提高诱导型CreER的 … culligan faucet drinking water filterWebGT1-1小鼠垂体瘤细胞培养注意事项. 1. 收到细胞后首先观察细胞瓶是否完好，培养液是否有漏液、浑浊等现象，若有上述现 象发生请及 时和我们联系。. 2. 仔细阅读细胞说明书， … culligan faucet filter chrome